Hemagglutinin stalk-based monoclonal antibody elicits broadly reactivity against group 1 influenza A virus.

ABSTRACT:

Background

Influenza virus remains a continuous and severe threat to public health worldwide, and its prevention and treatment have always been a major international issue. Because of its ability to evade immune surveillance through rapid antigenic drift and antigenic shift, broad-spectrum vaccines seem increasingly important.

Methods

A mAb named 3C12 from an immortalized hybrid cell was generated via immunizing mice with HA2 protein from A/chicken/Anhui/BRI99/2016 (AH/BRI99/16, H9N2) generated by prokaryotic expression. Then, its broad-spectrum activity was analyzed by WB and IFA. Next, the minimal linear epitope was identified via analyzing the reaction of a series of HA truncations with 3C12. Finally, the protective effects of 3C12 were evaluated in vitro and in vivo infe

SUBMITTER: Huang J 

PROVIDER: S-EPMC7720065 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature