Identification of a cytosine methyltransferase that improves transformation efficiency in Methylomonas sp. DH-1.

ABSTRACT:

Background

Industrial biofuels and other value-added products can be produced from metabolically engineered microorganisms. Methylomonas sp. DH-1 is a candidate platform for bioconversion that uses methane as a carbon source. Although several genetic engineering techniques have been developed to work with Methylomonas sp. DH-1, the genetic manipulation of plasmids remains difficult because of the restriction-modification (RM) system present in the bacteria. Therefore, the RM system in Methylomonas sp. DH-1 must be identified to improve the genetic engineering prospects of this microorganism.

Results

We identified a DNA methylation site, TGGCCA, and its corresponding cytosine methyltransferase for the first time in Methylomonas sp. DH-1 through whole-genome bisulfite sequenci

SUBMITTER: Ren J 

PROVIDER: S-EPMC7720504 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature