Linkage mapping and QTL analysis of flowering time using ddRAD sequencing with genotype error correction in Brassica napus.

ABSTRACT:

Background

Brassica napus is an important oilseed crop cultivated worldwide. During domestication and breeding of B. napus, flowering time has been a target of selection because of its substantial impact on yield. Here we use double digest restriction-site associated DNA sequencing (ddRAD) to investigate the genetic basis of flowering in B. napus. An F₂ mapping population was derived from a cross between an early-flowering spring type and a late-flowering winter type.

Results

Flowering time in the mapping population differed by up to 25 days between individuals. High genotype error rates persisted after initial quality controls, as suggested by a genotype discordance of ~ 12% between biological sequencing replicates. After genotype error correction, a linkage map

SUBMITTER: Scheben A 

PROVIDER: S-EPMC7720618 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature