Microarray analysis identifies defects in regenerative and immune response pathways in COPD airway basal cells.

ABSTRACT:

Background

Airway basal cells are specialised stem cells and regenerate airway epithelium. Airway basal cells isolated from patients with COPD regenerate airway epithelium with an abnormal phenotype. We performed gene expression analysis to gain insights into the defective regenerative programme in COPD basal cells.

Methods

We conducted microarray analysis and compared COPD versus normal basal cells to identify differentially regulated genes (DEGs) and the enriched biological pathways. We determined the correlation of DEGs with cell polarisation and markers of ciliated and goblet cells. HOXB2 was knocked down in 16HBE14o- cells and monitored for polarisation of cells. HOXB2 expression in the lung sections was determined by immunofluorescence.

Results<

SUBMITTER: Pineau F 

PROVIDER: S-EPMC7720690 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature