Association of PARP1-specific polymorphisms and haplotypes with non-small cell lung cancer subtypes.

ABSTRACT:

Objective

The carcinogenesis role of PARP1 in lung cancer is still not clear. Analysis at allelic levels cannot fully explain the function of PARP1 on lung cancer. Our study aims to further explore the relation between PARP1 haplotypes and lung cancer.

Materials and methods

DNA and RNA were extracted from non-small cell lung cancer (NSCLC) tumor and adjacent normal fresh frozen tissue. Five PARP1-SNPs were genotyped and PARP1-specific SNPs were imputed using IMPUTE and SHAPEIT software. The SNPs were subjected to allelic, haplotype and SNP-SNP interaction analyses. Correlation between SNPs and mRNA/protein expressions were performed.

Results

SNP imputation inferred the ungenotyped SNPs and increased the power for association analysis. Tumor tissue samples are more lik

SUBMITTER: Jin J 

PROVIDER: S-EPMC7721167 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature