Adoptive Immunotherapy Based on Chain-Centric TCRs in Treatment of Infectious Diseases.

ABSTRACT: Complications after vaccination, lack of vaccines against certain infections, and the emergence of antibiotic-resistant microorganisms point to the need for alternative ways of protection and treatment of infectious diseases. Here, we proposed a therapeutic approach to control salmonellosis based on adoptive cell therapy. We showed that the T cell receptor (TCR) repertoire of salmonella-specific memory cells contains 20% of TCR variants with the dominant-active α-chain. Transduction of intact T lymphocytes with the dominant salmonella-specific TCRα led to their enhanced in vitro proliferation in response to salmonella. Adoptive transfer of transduced T cells resulted in a significant decrease in bacterial loads in mice infected with salmonella before or after the adoptive transfer.

SUBMITTER: Kalinina AA 

PROVIDER: S-EPMC7721641 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature