Segment-specific optogenetic stimulation in Drosophila melanogaster with linear arrays of organic light-emitting diodes.

ABSTRACT: Optogenetics allows light-driven, non-contact control of neural systems, but light delivery remains challenging, in particular when fine spatial control of light is required to achieve local specificity. Here, we employ organic light-emitting diodes (OLEDs) that are micropatterned into linear arrays to obtain precise optogenetic control in Drosophila melanogaster larvae expressing the light-gated activator CsChrimson and the inhibitor GtACR2 within their peripheral sensory system. Our method allows confinement of light stimuli to within individual abdominal segments, which facilitates the study of larval behaviour in response to local sensory input. We show controlled triggering of specific crawling modes and find that targeted neurostimulation in abdominal segments switches the direction

SUBMITTER: Murawski C 

PROVIDER: S-EPMC7721879 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature