Baculovirus-free insect cell expression system for high yield antibody and antigen production.

ABSTRACT: Antibodies are essential tools for therapy and diagnostics. Yet, production remains expensive as it is mostly done in mammalian expression systems. As most therapeutic IgG require mammalian glycosylation to interact with the human immune system, other expression systems are rarely used for production. However, for neutralizing antibodies that are not required to activate the human immune system as well as antibodies used in diagnostics, a cheaper production system would be advantageous. In our study, we show cost-efficient, easy and high yield production of antibodies as well as various secreted antigens including Interleukins and SARS-CoV-2 related proteins in a baculovirus-free insect cell expression system. To improve yields, we optimized the expression vector, media and feeding strateg

SUBMITTER: Korn J 

PROVIDER: S-EPMC7721901 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature