Construction of a mApple-D6A3-mediated biosensor for detection of heavy metal ions.

ABSTRACT: Pollution of heavy metals in agricultural environments is a growing problem to the health of the world's human population. Green, low-cost, and efficient detection methods can help control such pollution. In this study, a protein biosensor, mApple-D6A3, was built from rice-derived Cd²⁺-binding protein D6A3 fused with the red fluorescent protein mApple at the N-terminus to detect the contents of heavy metals. Fluorescence intensity of mApple fused with D6A3 indicated the biosensor's sensitivity to metal ions and its intensity was more stable under alkaline conditions. mApple-D6A3 was most sensitive to Cu²⁺, then Ni²⁺, then Cd²⁺. Isothermal titration calorimetry experiments demonstrated that mApple-D6A3 successfully bound to each of these three met

SUBMITTER: Ji Y 

PROVIDER: S-EPMC7721944 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature