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Remediation of oily sludge wastes using biosurfactant produced by bacterial isolate Pseudomonas balearica strain Z8.

ABSTRACT: Biological treatment of oily sludge wastes was studied using an isolated halo-tolerant strain Pseudomonas balearica strain Z8. An oily sludge sample was obtained from oil fields of south waste of Iran and was fully characterized. The initial TPH content was 44,500 mg kg^-1. The ability of Pseudomonas balearica strain Z8 in production of biosurfactant was investigated using oil displacement method. Results demonstrated that isolated strain is a biosurfactant producing bacteria. The CMC and emulsification index [E₂₄] of produced biosurfactant were 90 mg L^-1 and 44% for crude oil. Effect of operational parameters including nitrogen source, sludge/water ratio and temperature were investigated against the time. The most TPH removal of 35% was observed for nitrogen source of NH₄Cl, sludge/ water ratio of 1:7 and temperature of 40 °C.

SUBMITTER: Nejad YS

PROVIDER: S-EPMC7721957 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Remediation of oily sludge wastes using biosurfactant produced by bacterial isolate Pseudomonas balearica strain Z8.

Nejad Yaser Soltani YS Jaafarzadeh Neematollah N Ahmadi Mehdi M Abtahi Mehrnoosh M Ghafari Shokouh S Jorfi Sahand S

Journal of environmental health science & engineering 20200509 2

Biological treatment of oily sludge wastes was studied using an isolated halo-tolerant strain Pseudomonas balearica strain Z8. An oily sludge sample was obtained from oil fields of south waste of Iran and was fully characterized. The initial TPH content was 44,500 mg kg-1. The ability of Pseudomonas balearica strain Z8 in production of biosurfactant was investigated using oil displacement method. Results demonstrated that isolated strain is a biosurfactant producing bact ...[more]

PMID: 33312581

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Project description:BackgroundBiosurfactants, being highly biodegradable, ecofriendly and multifunctional compounds have wide applications in various industrial sectors including environmental bioremediation. Surfactin, a member of lipopeptide family, which is considered as one of the most powerful biosurfactants due to its excellent emulsifying activities as well as environmental and therapeutic applications. Therefore, the aim of this study was to investigate the newly isolated bacterial strain S2MT for production of surfactin-like biosurfactants and their potential applications for oil-contaminated soil remediation.ResultsIn this study, the strain S2MT was isolated from lake sediment and was identified as Bacillus nealsonii based on transmitted electron microscopy (TEM) and 16S rRNA ribo-typing. The strain S2MT produced biosurfactant that reduced the surface tension (34.15 ± 0.6 mN/m) and displayed excellent emulsifying potential for kerosene (55 ± 0.3%). Additionally, the maximum biosurfactant product yield of 1300 mg/L was achieved when the composition of the culture medium was optimized through response surface methodology (RSM). Results showed that 2% glycerol and 0.1% NH4NO3 were the best carbon/nitrogen substrates for biosurfactant production. The parameters such as temperature (30 °C), pH (8), agitation (100 rpm), NH4NO3 (0.1%) and NaCl (0.5%) displayed most significant contribution towards surface tension reduction that resulted in enhanced biosurfactant yield. Moreover, the extracted biosurfactants were found to be highly stable at environmental factors such as salinity, pH and temperature variations. The biosurfactants were characterized as cyclic lipopeptides relating to surfactin-like isoforms (C13-C15) using thin-layer chromatography (TLC), Ultra high performance liquid chromatography and mass spectrometry (UHPLC-MS). The crude biosurfactant product displayed up to 43.6 ± 0.08% and 46.7 ± 0.01% remediation of heavy engine-oil contaminated soil at 10 and 40 mg/L concentrations, respectively.ConclusionPresent study expands the paradigm of surfactin-like biosurfactants produced by novel isolate Bacillus nealsonii S2MT for achieving efficient and environmentally acceptable soil remediation as compared to synthetic surfactants.

Dataset Information

Remediation of oily sludge wastes using biosurfactant produced by bacterial isolate Pseudomonas balearica strain Z8.

Publications

Remediation of oily sludge wastes using biosurfactant produced by bacterial isolate <i>Pseudomonas balearica strain Z8</i>.

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