The role of in utero endotoxin exposure in the development of inflammatory bowel disease in mice.

ABSTRACT:

Problem

Although early environmental influences are thought to influence the development of inflammatory bowel disease (IBD), little is known about the role of the in utero environment on subsequent IBD risk. We hypothesized that prenatal exposure to bacterial lipopolysaccharide (LPS) could modify the subsequent development of dextran sulfate sodium (DSS)-induced ulcerative colitis in adulthood by influencing the associated cellular and immune response.

Method of study

To test this hypothesis, we exposed developing mice in utero to LPS or saline (PBS) at E17.5, and then induced colitis at 5 weeks. We then assessed colitis severity and effects on the microbiome. In order to define the developmental impact of any potential LPS effect, we also exposed 1-week-old mice to either

SUBMITTER: Banfield E 

PROVIDER: S-EPMC7722031 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature