Maresin 1, a specialized proresolving mediator, stimulates intracellular [Ca²⁺ ] and secretion in conjunctival goblet cells.

ABSTRACT: Mucin secretion from conjunctival goblet cells forms the tear film mucin layer and requires regulation to function properly. Maresin 1 (MaR1) is a specialized proresolving mediator produced during the resolution of inflammation. We determined if MaR1 stimulates mucin secretion and signaling pathways used. Cultured rat conjunctival goblet cells were used to measure the increase in intracellular Ca²⁺ ([Ca²⁺ ]_i ) concentration and mucin secretion. MaR1-increased [Ca²⁺ ]_i and secretion were blocked by inhibitors of phospholipase C, protein kinase C, Ca²⁺ /calmodulin-dependent protein kinase II, and extracellular-regulated kinase 1/2. MaR1 added before addition of histamine counterregulated histamine-stimulated increas

SUBMITTER: Olsen MV 

PROVIDER: S-EPMC7722204 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature