Activation of protein kinase B by WNT4 as a regulator of uterine leiomyoma stem cell function.

ABSTRACT:

Objective

To investigate the functional interaction between the Wnt/β-catenin and protein kinase B (Akt) pathways in leiomyoma stem cells (LSC).

Design

Laboratory study.

Setting

Research laboratory.

Patient(s)

Premenopausal women (n = 36; age range: 28 to 49 years) undergoing hysterectomy or myomectomy for leiomyoma.

Intervention(s)

None.

Main outcome measure(s)

Gene expression, protein phosphorylation, and cell proliferation.

Result(s)

Cells from human leiomyoma tissues were sorted by fluorescence-activated cell sorting (FACS) into three populations: LSC, intermediate cells (LIC), and differentiated cells (LDC) with the function of the Wnt/β-catenin and Akt signaling pathways in leiomyoma cells evaluated using real-time quantitative polyme

SUBMITTER: Liu S 

PROVIDER: S-EPMC7722234 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature