Use of the Illumina EPIC methylation array for epigenomic research in the crab-eating macaque (Macaca fascicularis).

ABSTRACT:

Background

Commercially available Illumina DNA methylation arrays (HumanMethylation 27K, HumanMethylation450, and MethylationEPIC BeadChip) can be used for comprehensive DNA methylation analyses of not only the human genome but also other mammalian genomes, ranging from those of nonhuman primates to those of rodents. However, practical application of the EPIC array to the crab-eating macaque has not been reported.

Methods

Through bioinformatic analyses involving cross-species comparison and consideration of probe performance, we selected array probes that can be reliably used for the crab-eating macaque genome. A DNA methylation assay using an EPIC array was performed on genomic DNA extracted from the brains of five crab-eating macaques. The obtained DNA methylation data wer

SUBMITTER: Nakachi Y 

PROVIDER: S-EPMC7722662 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature