Deep sequencing detects human papillomavirus (HPV) in cervical cancers negative for HPV by PCR.

ABSTRACT:

Background

Human papillomavirus (HPV) is a necessary cause of cervical cancer, although some invasive cervical cancers may test negative by HPV PCR. We previously requested all invasive cervical cancers in Sweden during 10 years and subjected them to PCR. We also optimised methods for deep sequencing of formalin-fixed paraffin-embedded samples.

Methods

Using Novaseq 6000, we simultaneously sequenced total DNA and cDNA from 392 HPV PCR-negative cervical cancers. Non-human reads were queried against all known HPVs. The complete database now contains PCR and/or deep sequencing data on 2850 invasive cervical cancers.

Results

HPV sequences were detected in 169/392 of HPV PCR-negative cervical cancers. Overall, 30 different HPV types were detected, but only 5 types were pre

SUBMITTER: Arroyo Muhr LS 

PROVIDER: S-EPMC7722749 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature