Transcriptional profiling of murine retinas undergoing semi-synchronous cone photoreceptor differentiation.

ABSTRACT: Uncovering the gene regulatory networks that control cone photoreceptor formation has been hindered because cones only make up a few percent of the retina and form asynchronously during development. To overcome these limitations, we used a γ-secretase inhibitor, DAPT, to disrupt Notch signaling and force proliferating retinal progenitor cells to rapidly adopt neuronal identity. We treated mouse retinal explants at the peak of cone genesis with DAPT and examined tissues at several time-points by histology and bulk RNA-sequencing. We found that this treatment caused supernumerary cone formation in an overwhelmingly synchronized fashion. This analysis revealed several categorical patterns of gene expression changes over time relative to DMSO treated control explants. These were placed in the

SUBMITTER: Kaufman ML 

PROVIDER: S-EPMC7722777 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature