Nuclear numbers in syncytial muscle fibers promote size but limit the development of larger myonuclear domains.

ABSTRACT: Mammalian cells exhibit remarkable diversity in cell size, but the factors that regulate establishment and maintenance of these sizes remain poorly understood. This is especially true for skeletal muscle, comprised of syncytial myofibers that each accrue hundreds of nuclei during development. Here, we directly explore the assumed causal relationship between multinucleation and establishment of normal size through titration of myonuclear numbers during mouse neonatal development. Three independent mouse models, where myonuclear numbers were reduced by 75, 55, or 25%, led to the discovery that myonuclei possess a reserve capacity to support larger functional cytoplasmic volumes in developing myofibers. Surprisingly, the results revealed an inverse relationship between nuclei numbers and rese

SUBMITTER: Cramer AAW 

PROVIDER: S-EPMC7722938 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature