Design and Development of a Behaviorally Active Recombinant Neurotrophic Factor.

ABSTRACT:

Introduction

Carbamoylated erythropoietin (CEPO) is a chemically engineered, nonhematopoietic derivative of erythropoietin (EPO) that retains its antidepressant and pro-cognitive effects, which are attributed to the increased expression of neurotrophic factors like brain derived neurotrophic factor (BDNF), in the central nervous system. However, the chemical modification process which produces CEPO from erythropoietin (EPO) requires pure EPO as raw material, is challenging to scale-up and can also cause batch-to-batch variability. To address these key limitations while retaining its behavioral effects, we designed, expressed and analyzed a triple, glutamine, substitution recombinant mimetic of CEPO, named QPO.

Methods and materials

We employ a combination of computational st

SUBMITTER: Pekas NJ 

PROVIDER: S-EPMC7723032 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature