Cryo-EM structure of the calcium release-activated calcium channel Orai in an open conformation.

ABSTRACT: The calcium release-activated calcium channel Orai regulates Ca²⁺ entry into non-excitable cells and is required for proper immune function. While the channel typically opens following Ca²⁺ release from the endoplasmic reticulum, certain pathologic mutations render the channel constitutively open. Previously, using one such mutation (H206A), we obtained low (6.7 Å) resolution X-ray structural information on Drosophila melanogaster Orai in an open conformation (Hou et al., 2018). Here we present a structure of this open conformation at 3.3 Å resolution using fiducial-assisted cryo-electron microscopy. The improved structure reveals the conformations of amino acids in the open pore, which dilates by outward movements of subunits. A ring of phenylalanine residues

SUBMITTER: Hou X 

PROVIDER: S-EPMC7723414 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature