Lactobacillus rhamnosus attenuates intestinal inflammation induced by Fusobacterium nucleatum infection by restoring the autophagic flux.

ABSTRACT: Autophagy plays a dual role in the responses to the gut microflora. The present study aimed to examine the effects of Lactobacillus rhamnosus (L. rhamnosus) on Fusobacterium nucleatum (F. nucleatum)‑induced intestinal dysfunction and to elucidate the underlying mechanisms, with particular focus on autophagy. Inflammatory models were established by treatment with L. rhamnosus following F. nucleatum intervention using cells or a mouse model of dextran sulfate sodium (DSS)‑induced acute colitis. Autophagosomes were visualized by confocal microscopy following transfection with a tandem GFP‑mCherry‑LC3 construct and also by transmission electron microscopy. Autophagy‑associated protein levels were examined by western blot analysis and immunohistochemistry. It was observed that F. nucleatum indu

SUBMITTER: Duan C 

PROVIDER: S-EPMC7723500 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature