A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing.

ABSTRACT: Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR-Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors.

SUBMITTER: Grunewald J 

PROVIDER: S-EPMC7723518 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature