Screening and identification of a CD44v6 specific peptide using improved phage display for gastric cancer targeting.

ABSTRACT:

Background

Peptide probes can be applied for biomarker targeting to improve the diagnostic accuracy. Cluster of differentiation 44 (CD44) is up-regulated in gastric cancer (GC). Among all the variants of CD44, CD44v6 is reported the most promising biomarker for GC. The purpose of this study was generating and identification a peptide ligand specific to CD44v6.

Methods

A 12-mer phage peptide library was screened on CD44v overexpressed HEK-293 cells with an improved subtractive method. Five candidate sequences emerged. Candidate phages were selected using enzyme-linked immunosorbent assay and competitive inhibition assays. Then the sequence (designated ELT) was chosen for further study. Its binding affinity and specificity were verified on recombinant protein, GC cells, GC tis

SUBMITTER: Zhang D 

PROVIDER: S-EPMC7723568 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature