Profiling circulating T follicular helper cells and their effects on B cells in post-cardiac transplant recipients.

ABSTRACT:

Background

To evaluate circulating T follicular helper (cTfh) cells and characterize their function in chronic-phase recipients after heart transplantation.

Methods

Participants were divided into healthy control (HC, n=40), preoperative (Pre, n=40), and post-transplantation chronic-phase recipient (1-year, n=40) groups. The percentages of cTfh cell subsets and CD19⁺ B cell subsets were measured using flow cytometry. In vitro co-culture experiments were performed using cTfh cells and B cells isolated by fluorescence-activated cell sorting. Plasma concentrations of IL-21, chemokine ligand 13 (CXCL13), immunoglobulin G1 (IgG1), and immunoglobulin G3 (IgG3) were quantified using enzyme-linked immunosorbent assays (ELISA).

Results

cTfh and programmed cell

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7723658 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature