Analysis of the virome associated to grapevine downy mildew lesions reveals new mycovirus lineages.

ABSTRACT: The obligatory biotrophic oomycetes Plasmopara viticola is the causal agent of downy mildew, a destructive disease of grapevine worldwide. So far, chemical fungicides are widely employed to limit this pathogen, but their adverse effects are stimulating the quest for environmentally friendly alternative approaches. Here, we report on the search for mycoviruses that might be later developed as biocontrol agents for this pathogen. Symptomatic leaves were collected from various regions in Spain and Italy and mycelia associated to leaf lesions was harvested. Total RNA extractions were depleted of rRNA and metatranscriptomes were generated using a high-throughput sequencing approach. The virome associated to leaf lesions was then characterized through a bioinformatic pipeline relying on b

SUBMITTER: Chiapello M 

PROVIDER: S-EPMC7724247 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature