Developmental regulation of Dirofilaria immitis microfilariae and evaluation of ecdysone signaling pathway transcript level using droplet digital PCR.

ABSTRACT:

Background

Current measures for the prevention of dirofilariasis, caused by the dog heartworm, Dirofilaria immitis, rely on macrocyclic lactones, but evidence of drug-resistant isolates has called for alternative approaches to disease intervention. As microfilariae are known to be in a state of developmental arrest in their mammalian host and then undergo two molts once inside the arthropod, the aim of this study was to look at the developmental regulation of D. immitis microfilariae that occurs in their arthropod host using in vitro approaches and to investigate the role of the ecdysone signaling system in this development regulation.

Methods

Dirofilaria immitis microfilariae extracted from dog blood were incubated under various culture conditions to identify those most sui

SUBMITTER: Shang Kuan TC 

PROVIDER: S-EPMC7724712 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature