Novel stromal biomarker screening in pancreatic cancer patients using the in vitro cancer-stromal interaction model.

ABSTRACT:

Background

Stromal fibroblasts associated with pancreatic ductal adenocarcinoma (PDAC) play an important role in tumor progression through interactions with cancer cells. Our proposed combination strategies of in vitro and in silico biomarker screening through a cancer-stromal interaction model were previously identified several actin-binding proteins in human colon cancer stroma. The main aim of the present study was to identify novel prognostic markers in human PDAC stroma using our strategies.

Methods

Five primary cultivated fibroblasts from human pancreas were stimulated by two types of pancreatic cancer-cell-conditioned medium (Capan-1 and MIA PaCa-2) followed by gene expression analysis to identify up-regulated genes. Publicly available microarray data set concomitant

SUBMITTER: Nishida Y 

PROVIDER: S-EPMC7724826 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature