Dissecting molecular mechanisms underlying H₂O₂-induced apoptosis of mouse bone marrow mesenchymal stem cell: role of Mst1 inhibition.

ABSTRACT:

Background

Bone marrow mesenchymal stem cell (BM-MSC) has been shown to treat pulmonary arterial hypertension (PAH). However, excessive reactive oxygen species (ROS) increases the apoptosis of BM-MSCs, leading to poor survival and engraft efficiency. Thus, improving the ability of BM-MSCs to scavenge ROS may considerably enhance the effectiveness of transplantation therapy. Mammalian Ste20-like kinase 1 (Mst1) is a pro-apoptotic molecule which increases ROS production. The aim of this study is to uncover the underlying mechanisms the effect of Mst1 inhibition on the tolerance of BM-MSCs under H₂O₂ condition.

Methods

Mst1 expression in BM-MSCs was inhibited via transfection with adenoviruses expressing a short hairpin (sh) RNA directed against Mst1 (Ad-s

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC7724846 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature