MAFG-AS1 aggravates the progression of pancreatic cancer by sponging miR-3196 to boost NFIX.

ABSTRACT:

Background

A host of researches have demonstrated the regulation of long non-coding RNAs (lncRNAs) in the progression of pancreatic cancers (PC). In this study, our main task was to analyze the function of MAF bZIP transcription factor G antisense RNA 1 (MAFG-AS1) in PC.

Methods

RT-qPCR measured gene expression. Functional experiments, including EdU assay, flow cytometry analysis, TUNEL assay and transwell assay, assessed the biological changes of PC cells. RNA pull down assay, luciferase reporter assay and RIP assay verified the interaction between RNAs.

Results

MAFG-AS1 was lowly expressed in normal pancreatic samples but up-regulated in PC tissues and cell lines. Besides, MAFG-AS1 silence suppressed cell proliferation and migration whereas promoted cell apoptosis i

SUBMITTER: Ye L 

PROVIDER: S-EPMC7724861 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature