Mechanisms of Theta Plasmid Replication in Enterobacteria and Implications for Adaptation to Its Host.

ABSTRACT: Plasmids are autonomously replicating sequences that help cells adapt to diverse stresses. Theta plasmids are the most frequent plasmid class in enterobacteria. They co-opt two host replication mechanisms: replication at oriC, a DnaA-dependent pathway leading to replisome assembly (theta class A), and replication fork restart, a PriA-dependent pathway leading to primosome assembly through primer extension and D-loop formation (theta classes B, C, and D). To ensure autonomy from the host's replication and to facilitate copy number regulation, theta plasmids have unique mechanisms of replication initiation at the plasmid origin of replication (ori). Tight plasmid copy number regulation is essential because of the major and direct impact plasmid gene dosage has on gene expressio

SUBMITTER: Kim JW 

PROVIDER: S-EPMC7724965 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature