Functional and Sustainable 3D Human Neural Network Models from Pluripotent Stem Cells.

ABSTRACT: Three-dimensional in vitro cell culture models, particularly for the central nervous system, allow for the exploration of mechanisms of organ development, cellular interactions, and disease progression within defined environments. Here we describe the development and characterization of three-dimensional tissue models that promote the differentiation and long-term survival of functional neural networks. These tissue cultures show diverse cell populations including neurons and glial cells (astrocytes) interacting in 3D with spontaneous neural activity confirmed through electrophysiological recordings and calcium imaging over at least 8 months. This approach allows for the direct integration of pluripotent stem cells into the 3D construct bypassing early neural differentiation steps (embryoi

SUBMITTER: Cantley W 

PROVIDER: S-EPMC7725274 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature