An Integrated Workflow for Global, Glyco-, and Phospho-proteomic Analysis of Tumor Tissues.

ABSTRACT: Recently, the rapid development and application of mass spectrometry (MS)-based technologies have markedly improved the comprehensive proteomic characterization of global proteome and protein post-translational modifications (PTMs). However, the current conventional approach for global proteomic analysis is often carried out separately from PTM analysis. In our study, we developed an integrated workflow for multiplex analysis of global, glyco-, and phospho-proteomics using breast cancer patient-derived xenograft (PDX) tumor samples. Our approach included the following steps: trypsin-digested tumor samples were enriched for phosphopeptides through immobilized metal ion affinity chromatography (IMAC), followed by enrichment of glycopeptides through mixed anion exchange (MAX) method, and then

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC7725359 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature