Digital-WGS: Automated, highly efficient whole-genome sequencing of single cells by digital microfluidics.

ABSTRACT: Single-cell whole-genome sequencing (WGS) is critical for characterizing dynamic intercellular changes in DNA. Current sample preparation technologies for single-cell WGS are complex, expensive, and suffer from high amplification bias and errors. Here, we describe Digital-WGS, a sample preparation platform that streamlines high-performance single-cell WGS with automatic processing based on digital microfluidics. Using the method, we provide high single-cell capture efficiency for any amount and types of cells by a wetted hydrodynamic structure. The digital control of droplets in a closed hydrophobic interface enables the complete removal of exogenous DNA, sufficient cell lysis, and lossless amplicon recovery, achieving the low coefficient of variation and high coverage at multiple scales.

SUBMITTER: Ruan Q 

PROVIDER: S-EPMC7725457 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature