Species Quantification in Complex Herbal Formulas-Vector Control Quantitative Analysis as a New Method.

ABSTRACT: Product mislabeling and/or species fraud in Traditional Chinese Medicine (TCM) not only decrease TCM quality, but also pose a potential health issue to the end user. Up to now, methods to control TCM quality have been developed to detect specific metabolites or identify the original species. However, species quantification in complex herbal formulas is rarely concerned. Here, we reported a simple Vector Control Quantitative Analysis (VCQA) method for flexible and accurate multiplex species quantification in traditional Chinese herbal formulas. We developed PCR-based strategy to quickly generate the integrated DNA fragments from multiple targeted species, which can be assembled into the quantitative vector in one round of cloning by Golden Gate ligation and Gateway recombination technique.

SUBMITTER: Zhao B 

PROVIDER: S-EPMC7725679 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature