Critical review of methods for isothermal amplification of nucleic acids for environmental analysis.

ABSTRACT: The past 30 years have seen the emergence and proliferation of isothermal amplification methods (IAMs) for rapid, sensitive detection and quantification of nucleic acids in a variety of sample types. These methods share dependence on primers and probes with quantitative PCR, but they differ in the specific enzymes and instruments employed, and are frequently conducted in a binary, rather than quantitative format. IAMs typically rely on simpler instruments than PCR analyses due to the maintenance of a single temperature throughout the amplification reaction, which could facilitate deployment of IAMs in a variety of environmental and field settings. This review summarizes the mechanisms of the most common IAM methods and their use in studies of pathogens, harmful algae and fecal indicators i

SUBMITTER: Nieuwkerk DM 

PROVIDER: S-EPMC7726081 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature