Effective Biophysical Modeling of Cell Free Transcription and Translation Processes.

ABSTRACT: Transcription and translation are at the heart of metabolism and signal transduction. In this study, we developed an effective biophysical modeling approach to simulate transcription and translation processes. The model, composed of coupled ordinary differential equations, was tested by comparing simulations of two cell free synthetic circuits with experimental measurements generated in this study. First, we considered a simple circuit in which sigma factor 70 induced the expression of green fluorescent protein. This relatively simple case was then followed by a more complex negative feedback circuit in which two control genes were coupled to the expression of a third reporter gene, green fluorescent protein. Many of the model parameters were estimated from previous biophysical studies in

SUBMITTER: Adhikari A 

PROVIDER: S-EPMC7726328 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature