ELISA Test for the Serological Detection of Scedosporium/Lomentospora in Cystic Fibrosis Patients.

ABSTRACT: The detection and diagnosis of the opportunistic fungi Scedosporium spp. and Lomentospora prolificans still relies mainly on low-sensitive culture-based methods. This fact is especially worrying in Cystic Fibrosis (CF) patients in whom these fungal species are frequently isolated and may increase the risk of suffering from an infection or other health problems. Therefore, with the purpose of developing a serologic detection method for Scedosporium/Lomentospora, four different Scedosporium boydii protein extracts (whole cell protein extract, secretome, total cell surface and conidial surface associated proteins) were studied by ELISA to select the most useful for IgG detection in sera from CF patients. The four extracts were able to discriminate the Sce

SUBMITTER: Martin-Souto L 

PROVIDER: S-EPMC7726441 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature