CaMKIIα-Positive Interneurons Identified via a microRNA-Based Viral Gene Targeting Strategy.

ABSTRACT: Single-cell analysis is revealing increasing diversity in gene expression profiles among brain cells. Traditional promotor-based viral gene expression techniques, however, cannot capture the growing variety among single cells. We demonstrate a novel viral gene expression strategy to target cells with specific miRNA expression using miRNA-guided neuron tags (mAGNET). We designed mAGNET viral vectors containing a CaMKIIα promoter and microRNA-128 (miR-128) binding sites, and labeled CaMKIIα⁺ cells with naturally low expression of miR-128 (Lm128C cells) in male and female mice. Although CaMKIIα has traditionally been considered as an excitatory neuron marker, our single-cell sequencing results reveal that Lm128C cells are CaMKIIα⁺ inhibitory neurons of parvalbumin or som

SUBMITTER: Keaveney MK 

PROVIDER: S-EPMC7726537 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature