Cathepsin V suppresses GATA3 protein expression in luminal A breast cancer.

ABSTRACT:

Background

Lysosomal cysteine protease cathepsin V has previously been shown to exhibit elevated expression in breast cancer tissue and be associated with distant metastasis. Research has also identified that cathepsin V expression is elevated in tumour tissues from numerous other malignancies, but despite this, there has been limited examination of the function of this protease in cancer. Here we investigate the role of cathepsin V in breast cancer in order to delineate the molecular mechanisms by which this protease contributes to tumourigenesis.

Methods

Lentiviral transductions were used to generate shRNA cell line models, with cell line validation undertaken using RQ-PCR and Western blotting. Phenotypic changes of tumour cell biology were examined using clonogenic and in

SUBMITTER: Sereesongsaeng N 

PROVIDER: S-EPMC7726886 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature