CircAKT3 positively regulates osteogenic differentiation of human dental pulp stromal cells via miR-206/CX43 axis.

ABSTRACT:

Background

Human dental pulp stromal cells (hDPSCs) are promising sources of mesenchymal stem cells (MSCs) for bone tissue regeneration. Circular RNAs (circRNAs) have been demonstrated to play critical roles in stem cell osteogenic differentiation. Herein, we aimed to investigate the role of circAKT3 during osteogenesis of hDPSCs and the underlying mechanisms of its function.

Methods

We performed circRNA sequencing to investigate the expression profiles of circular RNAs during osteogenesis of hDPSCs. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to detect the expression pattern of circAKT3 and miR-206 in hDPSCs during osteogenesis. We knocked down circAKT3 and interfered the expression of miR-206 to verify their regulatory role in hDPSC

SUBMITTER: Zhang B 

PROVIDER: S-EPMC7726914 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature