The interplay between ATF2 and NEAT1 contributes to lung adenocarcinoma progression.

ABSTRACT:

Background

Activating transcription factor 2 (ATF2), a member of the activator protein 1 (AP-1) transcription factor family, has been shown to be involved in the pathobiology of numerous cancers. However, the biological role and mechanism of ATF2 in lung adenocarcinoma (LUAD) remains to be elucidated.

Methods

The expression of ATF2, NEAT1 and miR-26a-5p in LUAD tissues and cell lines was detected by qRT-PCR and western blotting. The interaction between ATF2, NEAT1, and miR-26a-5p was validated by chromatin immunoprecipitation, luciferase reporter assay and RNA immunoprecipitation. Cell proliferation, invasion and tumorigenesis of LUAD cells were analyzed by using CCK8, transwell invasion assay and xenograft tumor model.

Results

We confirmed that ATF2 expression was in

SUBMITTER: Liu J 

PROVIDER: S-EPMC7727147 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature