Therapeutic potential of human induced pluripotent stem cells and renal progenitor cells in experimental chronic kidney disease.

ABSTRACT:

Background

Chronic kidney disease (CKD) is a global public health problem. Cell therapy using pluripotent stem cells represents an attractive therapeutic approach for the treatment of CKD.

Methods

We transplanted mitomycin C (MMC)-treated human induced pluripotent stem cells (hiPSCs) and renal progenitor cells (RPCs) into a CKD rat model system. The RPC and hiPSC cells were characterized by immunofluorescence and qRT-PCR. Untreated 5/6 nephrectomized rats were compared to CKD animals receiving the same amount of MMC-treated hiPSCs or RPCs. Renal function, histology, and immunohistochemistry were evaluated 45 days post-surgery.

Results

We successfully generated hiPSCs from peripheral blood and differentiated them into RPCs expressing renal progenitor genes (PAX2, WT1,

SUBMITTER: Ribeiro PC 

PROVIDER: S-EPMC7727202 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature