Composition and origin of lung fluid proteome in premature infants and relationship to respiratory outcome.

ABSTRACT:

Background

Infants born at extremely low gestational age are at high risk for bronchopulmonary dysplasia and continuing lung disease. There are no early clinical biomarkers for pulmonary outcome and limited therapeutic interventions.

Objectives

We performed global proteomics of premature infant tracheal aspirate (TA) and plasma to determine the composition and source of lung fluid proteins and to identify potential biomarkers of respiratory outcome.

Methods

TA samples were collected from intubated infants in the TOLSURF cohort before and after nitric oxide treatment, and plasma was collected from NO CLD infants. Protein abundance was assayed by HPLC/tandem mass spectrometry and Protein Prospector software. mRNA abundance in mid-gestation fetal lung was assessed by RNA

SUBMITTER: Ballard PL 

PROVIDER: S-EPMC7728257 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature