Precise determination of input-output mapping for multimodal gene circuits using data from transient transfection.

ABSTRACT: The mapping of molecular inputs to their molecular outputs (input/output, I/O mapping) is an important characteristic of gene circuits, both natural and synthetic. Experimental determination of such mappings for synthetic circuits is best performed using stably integrated genetic constructs. In mammalian cells, stable integration of complex circuits is a time-consuming process that hampers rapid characterization of multiple circuit variants. On the other hand, transient transfection is quick. However, it is an extremely noisy process and it is unclear whether the obtained data have any relevance to the input/output mapping of a circuit obtained in the case of a stable integration. Here we describe a data processing workflow, Peakfinder algorithm for flow cytometry data (PFAFF), that allows

SUBMITTER: Stelzer C 

PROVIDER: S-EPMC7728399 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature