Persulfidation of transcription factor FOXO1 at cysteine 457: A novel mechanism by which H₂S inhibits vascular smooth muscle cell proliferation.

ABSTRACT:

Introduction

The proliferation of vascular smooth muscle cells (VSMCs) is an important physiological and pathological basis for many cardiovascular diseases. Endogenous hydrogen sulfide (H₂S), the third gasotransmitter, is found to preserve vascular structure by inhibiting VSMC proliferation. However, the mechanism by which H₂S suppresses VSMC proliferation has not been fully clear.

Objectives

This study aimed to explore whether H₂S persulfidates the transcription factor FOXO1 to inhibit VSMC proliferation.

Methods

After the proliferation of VSMC A7r5 cells was induced by endothelin-1 (ET-1), FOXO1 phosphorylation and proliferating cell nuclear antigen (PCNA) expression were detected by Western blotting, the degree of FOXO1 nuclear exc

SUBMITTER: Tian X 

PROVIDER: S-EPMC7728583 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature