Gut Microbiome Contributes to Liver Fibrosis Impact on T Cell Receptor Immune Repertoire.

ABSTRACT: Gut microbiota (GM) modifies the intrahepatic immune microenvironment, but the underlying mechanisms remain poorly understood. Liver fibrosis-associated imprinting is predicted to be reflected in GM. This study investigated the link between GM and the intrahepatic T cell receptor (TCR) immune repertoire (IR), and whether GM modulates the intrahepatic immune microenvironment via TCR IR during liver fibrosis. We analyzed the correlation between GM and TCR IR during liver fibrogenesis. Accordingly, 16S rRNA gene sequencing (16S-seq) and bulk immune repertoire sequencing (IR-seq) were performed to characterize GM and intrahepatic TCR IR. Fecal microbial transplant (FMT) and TCRβ knockout (Tcrb^KO) mouse models were employed to determine the biological link between GM and TCR IR in li

SUBMITTER: Liang Q 

PROVIDER: S-EPMC7729130 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature