Antimicrobial effects of nitric oxide in murine models of Klebsiella pneumonia.

ABSTRACT:

Rationale

Inhalation of nitric oxide (NO) exerts selective pulmonary vasodilation. Nitric oxide also has an antimicrobial effect on a broad spectrum of pathogenic viruses, bacteria and fungi.

Objectives

The aim of this study was to investigate the effect of inhaled NO on bacterial burden and disease outcome in a murine model of Klebsiella pneumonia.

Methods

Mice were infected with Klebsiella pneumoniae and inhaled either air alone, air mixed with constant levels of NO (at 80, 160, or 200 parts per million (ppm)) or air intermittently mixed with high dose NO (300 ppm). Forty-eight hours after airway inoculation, the number of viable bacteria in lung, spleen and blood was determined. The extent of infiltration of the lungs by inflammatory cells and the level of myeloper

SUBMITTER: Wiegand SB 

PROVIDER: S-EPMC7729265 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature