MUC16 affects the biological functions of ovarian cancer cells and induces an antitumor immune response by activating dendritic cells.

ABSTRACT:

Background

Ovarian cancer is the 5^th most common lethal gynecological malignancy with a 5-year survival rate of about 47% and a localized stage diagnosis of 15%, leading to about 125,000 global deaths each year. Therefore, it is urgent to explore novel and effective strategies for radical cure.

Methods

Short hairpin RNA targeting the Mucin16 (MUC16) gene was used to establish MUC16 knockdown in ovarian cancer cells. RT-PCR was performed to quantify the expression of MUC16 mRNA, and western blotting was performed to detect the expression of MUC16 and epithelial-mesenchymal transition-related proteins. Cell counting kit 8 (CCK8) wound healing and transwell assays were performed to assess cell proliferation and cell invasion. Flow c

SUBMITTER: Zhai Y 

PROVIDER: S-EPMC7729312 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature