Establishment of multiplex allele-specific blocker PCR for enrichment and detection of 4 common EGFR mutations in non-small cell lung cancer.

ABSTRACT:

Background

Lung cancer is one of the most severe cancers and the majority of patients miss the best timing for surgery when diagnosed, thus having to rely on radiotherapy, chemotherapy or target therapy. Epidermal growth factor receptor (EGFR) upregulation occurs in a large percentage of patients, who can then benefit from tyrosine kinase inhibitors (TKI). However, the EGFR mutations they carry will vary the effectiveness of TKI. Circulating tumor DNA (ctDNA) contains genetic information from cancer tissue that can be used as a liquid biopsy by non-invasive sampling. This study aimed to provide a solution for minor allele detection from ctDNA.

Methods

Our novel method, named multiplex allele-specific blocker PCR (MAB PCR), combines amplification refractory mutation system (A

SUBMITTER: Chen H 

PROVIDER: S-EPMC7729377 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature