Strand Displacement Activity of PrimPol.

ABSTRACT: Human PrimPol is a unique enzyme possessing DNA/RNA primase and DNA polymerase activities. In this work, we demonstrated that PrimPol efficiently fills a 5-nt gap and possesses the conditional strand displacement activity stimulated by Mn2+ ions and accessory replicative proteins RPA and PolDIP2. The DNA displacement activity of PrimPol was found to be more efficient than the RNA displacement activity and FEN1 processed the 5'-DNA flaps generated by PrimPol in vitro.

SUBMITTER: Boldinova EO 

PROVIDER: S-EPMC7729601 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature