Functional Validation of Glutaminesynthetase and Glutamatesynthase Genes in Durum Wheat near Isogenic Lines with QTL for High GPC.

ABSTRACT: Durum wheat (Triticum turgidum L. ssp. durum) is a minor crop grown on about 17 million hectares of land worldwide. Several grain characteristics determine semolina's high end-use quality, such as grain protein content (GPC) which is directly related to the final products' nutritional and technological values. GPC improvement could be pursued by considering a candidate gene approach. The glutamine synthetase (GS)/glutamate synthase (GOGAT) cycle represents a bottleneck in the first step of nitrogen assimilation. QTL for GPC have been located on all chromosomes, and several major ones have been reported on 2A and 2B chromosomes, where GS2 and Fd-GOGAT genes have been mapped. A useful and efficient method to validate a putative QTL is the constitution of near-isogenic lines (NILs) by using t

SUBMITTER: Nigro D 

PROVIDER: S-EPMC7730160 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature